RT PCR Full Form in Medical term is Reverse Transcription-Polymerase Chain Reaction. It’s a method for identifying viral RNA in living organisms. It is also applied to the investigation of viral genetics that many individuals may be infected with, like the herpes virus.
Initially, radioactive isotope markers were utilized to locate the intended genetic components. However, nowadays fluorescent dyes are used as refinery markers instead of isotopic labeling. This aids the scientists by providing feedback on their work in progress. But if we stick to tried-and-true RT-PCR techniques, then we won’t know the outcome until the very end of the procedure.
This real-time RT-PCR approach can be used to a number of different purposes. Several nations also employ these techniques in the search for more illnesses. To name only a few: Zika, COVID-19, Ebola, etc.
.It’s a method for creating duplicates of RNA and DNA. Using enzymes and nucleotides, this method can replicate a DNA sequence word for word. Reverse transcription is the term for this method.
The said polymerase chain reaction then uses primers and the proper enzymes to amplify this DNA strand.